ABSTRACT
Abstract INTRODUCTION: Loxoscelism is a clinical condition involving spiders of the genus Loxosceles. One of the most severe complications is acute kidney injury (AKI). This study aimed to investigate AKI and other complications associated with loxoscelism. METHODS: We analyzed cases diagnosed with loxoscelism in an area where most accidents were caused by Loxosceles amazonica from January 2010 to December 2015. AKI was defined according to the KDIGO criteria. RESULTS: Forty-five patients were recorded: 95.6% presented characteristic necrotic skin lesions and 13.3% AKI. CONCLUSIONS: Loxoscelism could cause kidney involvement which is uncommon and could lead to the death of these patients.
Subject(s)
Humans , Animals , Male , Female , Adult , Spider Bites/complications , Spider Venoms/toxicity , Phosphoric Diester Hydrolases/toxicity , Acute Kidney Injury/etiology , Brazil , Cross-Sectional StudiesABSTRACT
A doença de Chagas é um problema de saúde pública, afeta cerca de 6 milhões de pessoas emtodo o mundo e causa altos índices de morbidade e mortalidade nas populações afetadas. Obenzonidazol (BZ), fármaco utilizado no tratamento apresenta eficácia limitada e provoca sériosefeitos adversos. O transporte de fármacos por meio de nanopartículas tem demonstradoeficiência quanto à liberação controlada e direcionada de moléculas farmacológicas, além deapresentar propriedades desejáveis, como boa biocompatibilidade e biodegradabilidade. Oobjetivo do presente trabalho foi avaliar a citoxicidade de nanopartículas de benzonidazol emcarbonato de cálcio (BZ@CaCO3) sobre células de hospedeiras LLC-MK2; determinar o efeitotripanocida sobre as formas epimastigotas, tripomastigotas e amastigotas da cepa Y de T. cruzie comparar ao efeito do BZ, bem como, sugerir o mecanismo de morte. Os testes decitotoxicidade utilizando o método do MTT realizados com células LLC-MK2 cultivadas commeio DMEM a 10% de SBF demonstraram CI50 de 55 µg/mL para BZ@CaCO3 e 160 µg/mLpara BZ...
Chagas disease is a public health concern, affects about 6 million people worldwide and causeshigh morbidity and mortality in affected populations. One of the few drugs available fortreatment of this disease is Benznidazole (BZ) was developed over forty years ago, has limitedeffectiveness and cause severe adverse effects. The transport of drugs by nanoparticles hasattracted the interest of the scientific community as it demonstrates efficiency and the controlledand targeted release of pharmacological molecules, in addition to its desirable properties suchas good biocompatibility and biodegradability. The aim of this study was to evaluate thecytotoxicity of benznidazole nanoparticles (BZ@CaCO3) produced with calcium carbonate onLLC-MK2 host cells; to define the trypanocidal effect on epimastigotes, trypomastigote andamastigotes of Trypanosoma cruzi Y strain and compare the effect of BZ, as well as characterizethe nanoparticle-induced death mechanism. The cytotoxicity tests using the MTT methodperformed with LLC-MK2 cells grown in DMEM with 10% FBS showed IC50 of 55 µg/mL forBZ@CaCO3 and 160 µg/mL for BZ...